External Resources:

Illumina Platform Library Preparation and Sequencing

Library Construction

We offer the following library construction as standard services with set pricing:

  • TruSeq V2 mRNA
  • TruSeq Stranded mRNA
  • TruSeq Total RNA (Ribodepletion)
  • TruSeq Small RNA
  • KAPA DNA Library Preparation
  • Agilent SureSelect V4 Human Exome or Clinical Research Exome

We are more than happy to support other library preparation protocols, including low input DNA libraries, total RNA sequencing, targeted sequencing applications, 16S rRNA, amplicon sequencing, Nextera XT , etc. Please contact us with your specific needs.

Sequencing

HiSeq2500: We currently operate Illumina HiSeq instruments in both HighOut (V3 and V4 Chemistry) and Rapid Run Modes. Detailed specifications are available from Illumina.

HiSeq4000: Our most common run types are SR75, 10X (26X8X98) and PE100. Please inquire about other run types if you plan to submit fewer than 8 lanes.

NovaSeq 6000: We have an established partnership with UCSF to enable sequencing on their NovaSeq 6000 where advantageous. Please contact us for more information.

MiSeq: The MiSeq desktop sequencer is available for focused applications such as targeted gene sequencing, metagenomics, small genome sequencing, etc. Read lenths up to 600 bp (2X300) are currently supported.