Illumina Platform Library Preparation and Sequencing

Library Construction

We offer the following library construction as standard services with set pricing:

  • TruSeq V2 mRNA
  • Illumina Stranded mRNA Prep
  • Illumina Total RNA Prep (Ribodepletion)
  • TruSeq Small RNA
  • KAPA DNA Library Preparation
  • Agilent SureSelect Exome Sequencing Libraries

We may also support additional custom library preparation protocols, including low input DNA libraries, targeted sequencing applications, amplicon sequencing, Nextera XT, etc. Please contact us to discuss.

Sequencing

NovaSeq 6000 Sequencing:

The IGM Genomics Center now supports sequencing on the Illumina NovaSeq 6000. Please see available run configurations and proposed pricing below (subject to approval by rates committee).

Please be sure to acknowledge the NIH Shared Instrumentation Grant that funded the NovaSeq purchase:

“This publication includes data generated at the UC San Diego IGM Genomics Center utilizing an Illumina NovaSeq 6000 that was purchased with funding from a National Institutes of Health SIG grant (#S10 OD026929)

Please link your publications to the grant using My Bibliography: Read the NIH help pages on managing compliance using My NCBI

 

 

MiSeq: The MiSeq desktop sequencer is available for focused applications such as targeted gene sequencing, metagenomics, small genome sequencing, etc. Read lengths up to 600 bp (2X300) are currently supported.